https://doi.org/10.4081/jlimnol.2020.1946
Representative monitoring of the calcifying alga Phacotus lenticularis (Chlamydophyceae) in lentic ecosystems
Representative monitoring of calcifying algae
Submitted: 28 October 2019
Accepted: 15 December 2019
Published: 30 January 2020
Accepted: 15 December 2019
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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The biogenic carbonate precipitation by the freshwater alga Phacotus lenticularis may play a role in long-term carbon (C) fixation that has not yet been quantified. This is partly due to the absence of a standardised methodology to representatively sample and assess the cell density and sedimentation of P. lenticularis in lakes. The objective of the present study was to define an adequate sampling methodology taking into account the spatiotemporal variation of P. lenticularis as well as the sedimentation and dissolution of calcite shells. Simultaneous measurements in three different lake sub-basins of Lake Grosser Ostersee, Germany, showed that the spatial cell density of P. lenticularis was similar in each sub-basin. At all sites, the vertical P. lenticularis cell density maxima corresponded with the slowly downshifting thermocline from depths of 2 to 6 m. During the entire growth period, composite samples from 0 to 7 m included 89% of the total P. lenticularis population. Lake bathymetry, as well as external factors like wind exposure, did not appear to affect the abundance of these calcifying algae. Sediment traps at a depth below the thermocline (at 7 m) and 1 m above the lake bottom recorded sedimentation rates of P. lenticularis shell halves (sh) between 1.1×106 and 1.1×108 sh m−2 d−1, while mean cell concentrations in the water column were between 1.1×108 and 1.7×109 shells per m². Sinking velocity ranged between 3 and 4 m d−1. Sediment from traps installed at a depth of 7 m did not reflect mean shell concentrations in the water column above. Dissolution of carbonates reduced the number of shells in sediment traps at the lake bottom and during the storage of samples. A laboratory experiment showed that even distilled water used for dilution during microscopic analysis led to dissolution of P. lenticularis shells. In conclusion, combined sampling of P. lenticularis from open water and sediment traps close to the lake bottom delivers a most representative assessment of biogenic carbonate precipitation. Due to dissolution effects, high temporal resolution along with appropriate sample preservation are crucial, whereas spatial representativeness was already achieved with low number of sampling sites per lake.
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Edited by
Cristiana Callieri, CNR-IRSA Verbania, ItalySupporting Agencies
Bavarian State Ministry of the Environment and Consumer Protection, Project Nr. TLK10U-6627How to Cite
Lenz, Sebastian, Uta Raeder, and Juergen Geist. 2020. “Representative Monitoring of the Calcifying Alga <em>Phacotus lenticularis</Em> (Chlamydophyceae) in Lentic Ecosystems: Representative Monitoring of Calcifying Algae”. Journal of Limnology 79 (2). https://doi.org/10.4081/jlimnol.2020.1946.
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