Chlorophyll a interference in phycocyanin and allophycocyanin spectrophotometric quantification

Abstract

The accurate quantification of cyanobacteria phycobiliproteins is an important aspect in various research topics, such as cyanobacteria ecology and physiology studies, and especially to calibrate algorithms used in remote sensing of cyanobacterial blooms. Here we present a spectroscopic approach, exploiting spectrophotometric equations, aimed at improving the phycocyanin and allophycocyanin quantification when chlorophyll a is present in the phycobiliprotein aqueous extract.

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Author Biography

Rosaria Lauceri, National Research Council of Italy, Institute for the Study of Ecosystems

Institute for the Study of Ecosystems

Researcher

Published
2017-09-28
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Supporting Agencies
CARIPLO Fundation, University of Rome Tor Vergata, Department of Biology.
Keywords:
Cyanobacteria, phycocyanin, allophycocyanin, chlorophyll a, spectrophotometric equation.
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How to Cite
1.
Lauceri R, Bresciani M, Lami A, Morabito G. Chlorophyll a interference in phycocyanin and allophycocyanin spectrophotometric quantification. J Limnol [Internet]. 2017Sep.28 [cited 2020Jul.12];77(1). Available from: https://www.jlimnol.it/index.php/jlimnol/article/view/jlimnol.2017.1691