Spatially explicit genetic structure in the freshwater sponge Ephydatia fluviatilis (Linnaeus, 1759) within the framework of the monopolisation hypothesis

  • Livia Lucentini | livia@unipg.it Università di Perugia, Italy.
  • Lilia Gigliarelli Università di Perugia, Italy.
  • Maria Elena Puletti Università di Perugia, .
  • Antonella Palomba Università di Perugia, Italy.
  • Aurora Caldelli Università di Perugia, Italy.
  • Diego Fontaneto Istituto per lo Studio degli Ecosistemi. Consiglio Nazionale delle Ricerche, Italy.
  • Fausto Panara Dipartimento di Biologia Cellulare e Ambientale. Università degli Studi di Perugia, via Pascoli 1, 06123, Perugia. Italy., Italy.

Abstract

An apparent paradox is known for crustaceans, rotifers and bryozoans living in inland small water bodies: a potential for wide distribution due to the presence of resting stages is coupled with marked genetic differences between nearby water bodies, with enclave distributions masking clear phylogeographic patterns. According to the monopolisation hypothesis, this is due to the accumulation of resting stages, monopolising each water body. Freshwater sponges could represent a useful system to assess the generality of the mo- nopolisation hypothesis: these organisms i) live in the same habitats as crustaceans, rotifers and bryozoans, ii) produce resting stages that can accumulate, and iii) have indeed a wide distribution. Currently, no studies on spatially explicit genetic differentiation on fresh- water sponges are available. The aim of the present study is to provide additional empirical evidence in support of the generality of the scenario for small aquatic animals with resting stages by analysing genetic diversity at different spatial scales for an additional model system, the freshwater sponge ephydatia fluviatilis (Linnaeus, 1759). We expected that system genetic variability would follow enclave distributions, no clear phylogeographical patterns would be present, and nearby unconnected water bodies would show markedly different populations for this new model too. We analysed the ribosomal internal transcribed spacer regions 5.8S-ITS2-28S, the D3 domain of 28S subunit, the mitochondrial Cytochrome c Oxidase I (COI) and ten specific microsatellite markers of nine Italian and one Hungarian populations. Mitochondrial and nuclear sequences showed no or very low genetic polymorphism, whereas high levels of differentiation among populations and a significant polymorphism were observed using microsatellites. Microsatellite loci also showed a high proportion of private alleles for each population and an overall correlation between geographic and genetic distances among populations. All the expectations from the monopolisation hypothesis seemingly were confirmed for the analysed sponge.

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Author Biographies

Livia Lucentini, Università di Perugia
Dipartimento di Biologia Cellulare e Ambientale
Lilia Gigliarelli, Università di Perugia
Dipartimento di Biologia Cellulare e Ambientale.
Maria Elena Puletti, Università di Perugia
Dipartimento di Biologia Cellulare e Ambientale.
Antonella Palomba, Università di Perugia
Dipartimento di Specialità Medico Chirurgiche e Sanità Pubblica.
Aurora Caldelli, Università di Perugia
Dipartimento di Biologia Cellulare e Ambientale.
Published
2013-02-06
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Original Articles
Keywords:
Ephydatia fluviatilis, monopolisation hypothesis, microsatellites, 5.8-ITS2-28S, Cytochrome c Oxidase I
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How to Cite
1.
Lucentini L, Gigliarelli L, Puletti ME, Palomba A, Caldelli A, Fontaneto D, Panara F. Spatially explicit genetic structure in the freshwater sponge Ephydatia fluviatilis (Linnaeus, 1759) within the framework of the monopolisation hypothesis. J Limnol [Internet]. 2013Feb.6 [cited 2020Nov.30];72(1):e14. Available from: https://www.jlimnol.it/index.php/jlimnol/article/view/jlimnol.2013.e14