Spatial distribution of ammonia-oxidizing archaea and bacteria across eight freshwater lakes in sediments from Jiangsu of China

  • Xu Sun Nanjing University, China.
  • Aili Wang Nanjing University, China.
  • Liuyan Yang | yangly@nju.edu.cn Nanjing University, China.
  • Liyun Guo Nanjing University, China.
  • Qiankun Chen Nanjing University, China.
  • Zhinxin Hu Nanjing University, China.
  • Lijuan Jiang Nanjing University, China.
  • Lin Xiao Nanjing University, China.

Abstract

Ammonia-oxidizingarchaea (AOA) and ammonia-oxidizing bacteria (AOB) play an important role innitrogen transformation in freshwater sediments. However, it is still unclear towhat extent the distribution patterns of these microorganisms are affected bythe freshwater sediment across a large geographical scale. This study wasdesigned to gain insight into the heterogeneity distribution of AOA and AOB in32 freshwater sediments from a wide range of ecologic types. Real-time quantitative polymerasechain reaction PCR(qPCR) combined with the terminal restrictionfragment length polymorphism(T-RFLP) were employed to characterize the abundance, diversity, and communitystructure of the AOA and AOB in 32 freshwater sediments. AOA and AOB wereubiquitous in all sediments, and archaeal amoA far outnumbered bacterial amoA inmost sediments with lower organic matters. The abundance of AOA and AOB did notvary with the freshwater ecological type (macrophyte dominated region and algaedominated region). Based on  the T-RFLP of an amoA gene, this research found that organicmatters in pore water rather than other factors affect the AOA communitystructure in sediments, while the AOB were not significantly different in thefreshwater sediments. Phylogenetic analysis showed that all archaeal amoAsequences fell within either the Crenarchaeotal Group (CG) I.1b or the CGI.1asubgroup, and all AOB clustered with genus Nitrosomonas or Nitrosospira. The data obtained inthis study elucidates the role of ammonia-oxidizing archaea andammonia-oxidizing bacteria in the nitrogen cycle of freshwater ecosystems.

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Author Biographies

Xu Sun, Nanjing University
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment
Aili Wang, Nanjing University
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment
Liuyan Yang, Nanjing University
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment
Liyun Guo, Nanjing University
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment
Qiankun Chen, Nanjing University
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment
Zhinxin Hu, Nanjing University
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment
Lijuan Jiang, Nanjing University
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment
Lin Xiao, Nanjing University
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment
Published
2014-03-04
Section
Original Articles
Supporting Agencies
This work was supported by grants from the National Special Program of Water Environment (2012ZX07101-006) and National Basic Research Program of China (2008CB418102).
Keywords:
ammonia-oxidizing archaea, ammonia-oxidizing bacteria, trophic status, nitrification, macrozoobenthos, qPCR, T-RFLP
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How to Cite
1.
Sun X, Wang A, Yang L, Guo L, Chen Q, Hu Z, Jiang L, Xiao L. Spatial distribution of ammonia-oxidizing archaea and bacteria across eight freshwater lakes in sediments from Jiangsu of China. jlimnol [Internet]. 4Mar.2014 [cited 29Mar.2020];73(2). Available from: https://www.jlimnol.it/index.php/jlimnol/article/view/jlimnol.2014.905