Odonata assemblages in anthropogenically impacted lotic habitats

Submitted: 5 May 2020
Accepted: 7 October 2020
Published: 3 November 2020
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Increasing human pressures have a negative impact on freshwater habitats and their biota worldwide. To protect habitats and the species contained within them, ecological assessments over a gradient of near natural to degraded freshwater habitats are essential. Odonata assemblages were investigated at 46 study sites in Croatia encompassing slightly to heavily modified lowland rivers and streams. Nymphs were sampled between April and September 2016 using a benthos hand net. A total of 19 species was recorded, and Ischnura elegans (Vander Linden, 1820) and Platycnemis pennipes (Pallas, 1771) were most frequently recorded. RDA analysis indicated that water pollution (i.e. levels of chemical oxygen demand and total organic carbon), water temperature and oxygen concentration had the highest influence in the formation of Odonata assemblages at a specific habitat, reflecting their widely recognized bioindicator properties. This study showed that degraded lowland rivers can provide habitat for a relatively low number of species with broad ecological tolerance, while rare and specialist species are generally not able to reproduce there. These results contribute to our knowledge of Odonata occurrence in anthropogenically impacted habitats, and their relationships with such degraded environment.



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APHA, American Public Health Association, 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. Washington, DC.
AQEM expert consortium, 2002. Ecological classifications by AQEM expert consortium. www.aqem.de
AQEM consortium, 2002. Manual for the application of the AQEM method. A comprehensive method to assess European streams using benthic macroinvertebrates, developed for the purpose of the Water Framework Directive. Version 1.0, February 2002.
Arimoro FO, Chukwuji MAI, Ogheneghalome O, 2008. Effects of industrial waste water on the physical and chemical characteristics of a tropical coastal river. Res. J. Environ. Sci. 2:209-220. DOI: https://doi.org/10.3923/rjes.2008.209.220
Askew RR, 2004. The dragonflies of Europe. Second Edition, Harley Books, Essex: 308 pp.
Belančić A, Bogdanović T, Franković M, Ljuština M, Mihoković N, Vitas B, 2008. [Red book of Croatian Odonata].[Book in Croatian]. Ministry of Culture, State Department for Nature Protection, Zagreb: 132 pp.
Boeykens SP, Piol MN, Legal LS, Saralegui AB, Vázquez C, 2017. Eutrophication decrease: phosphate adsorption processes in presence of nitrates. J. Environ. Manag. 203:888–895. DOI: https://doi.org/10.1016/j.jenvman.2017.05.026
Boudot J-P, Kalkman VJ, 2015. Atlas of the European dragonflies and damselflies. KNNV publishers, Zeist: 381 pp.
Bried JT, Samways MJ, 2015. A review of odonatology in freshwater applied ecology and conservation science. Freshw. Sci. 34:1023-1031. DOI: https://doi.org/10.1086/682174
Brochard C, Groendijk D, van der Ploeg E, Termaat T, 2012. [Fotogids Larvenhuidjes van Libellen].[Book in Dutch]. KNNV publishers, Zeist: 320 pp.
Buczyński P, 2012. Dragonflies (Odonata) of the left-bank Bug River valley between Włodawa and Kodeń (middle-eastern Poland). Acta Biol. 19:47–69.
Butler RG, deMaynadier PG, 2007. The significance of littoral and shoreline habitat integrity to the conservation of lacustrine damselflies (Odonata). J. Insect Conserv. 12:23–36. DOI: https://doi.org/10.1007/s10841-006-9059-0
Carchini G, Rota E, 1985. Chemico-physical data on the habitats of rheophile Odonata from central Italy. Odonatologica 14:239–245.
Carpenter SR, Stanley EH, Vander Zanden MJ, 2011. State of the world's freshwater ecosystems: physical, chemical, and biological changes. Annu. Rev. Env. Resour. 36:75-99. DOI: https://doi.org/10.1146/annurev-environ-021810-094524
Chovanec A, 2018. Comparing and evaluating the dragonfly fauna (Odonata) of regulated and rehabilitated stretches of the fourth order metarhithron Gurtenbach (Upper Austria). Int. J. Odonatol. 21:15-32. DOI: https://doi.org/10.1080/13887890.2017.1409665
Chovanec A, Waringer J, 2001. Ecological integrity of river-floodplain systems assessment by dragonfly surveys (Insecta: Odonata). Regul. Rivers: Res. Manage. 17:493-507. DOI: https://doi.org/10.1002/rrr.664
Chovanec A, Schindler M, Waringer J, Wimmer R, 2015. The dragonfly association index (Insecta: Odonata) – a tool for the type-specific assessment of lowland river. River Res. Appl. 3:627-638. DOI: https://doi.org/10.1002/rra.2760
Clarke KR, Gorley RN, 2006. Primer V6: User Manual / Tutorial. Primer-E, Plymouth.
CORINE Land Cover Croatia, 2013. Croatian Environment and Nature Agency. Available from: http://www.haop.hr/hr/corine-land-cover-hrvatska-clc-hrvatska/corine-land-cover-hrvatska-clc-hrvatska
Corbet P, Brooks S, 2008. Dragonflies. Collins New Naturalist Library No 106. HarperCollins: London: 480 pp.
Dijkstra K-DB, Lewington R, 2006. Field Guide to the Dragonflies of Britain and Europe. British Wildlife Publishing, Gillingham: 320 pp.
Ferreras-Romero M, Márquez-Rodríguez J, Ruiz-García A, 2009. Implications of anthropogenic disturbance factors on the Odonata assemblage in a Mediterranean fluvial system. Int. J. Odonatol. 12:413-428. DOI: https://doi.org/10.1080/13887890.2009.9748354
Gerken B, Sternberg K, 1999. [Die Exuvien Europaïscher Libellen - The exuviae of European Dragonflies (Insecta, Odonata)].[Book in German]. Arnika & Eisvogel, Jena: 354 pp.
Giugliano L, Hardersen S, Santini G, 2012. Odonata communities in retrodunal ponds: a comparison of sampling methods. Int. J. Odonatol. 15:13-23. DOI: https://doi.org/10.1080/13887890.2012.660403
Golfieri B, Hardersen S, Maiolini B, Surian N, 2016. Odonates as indicators of the ecological integrity of the river corridor: development and application of the Odonate River Index (ORI) in northern Italy. Ecol. Indic. 61:234-247. DOI: https://doi.org/10.1016/j.ecolind.2015.09.022
Golfieri B, Surian N, Hardersen S, 2018. Towards a more comprehensive assessment of river corridor conditions: A comparison between the Morphological Quality Index and three biotic indices. Ecol. Indic. 84:525–534. DOI: https://doi.org/10.1016/j.ecolind.2017.09.011
Growns IO, Growns JE, 2001. Ecological effects of flow regulation on macroinvertebrate and periphytic diatom assemblages in the Hawkesbury-Nepean River, Australia. Regul. Rivers: Res. Manage. 17:275-293. DOI: https://doi.org/10.1002/rrr.622
Hacet N, Aktaç N, 2008. Two new records of Odonata (Gomphidae) for Turkey, Gomphus flavipes (Charpentier, 1825) and Ophiogomphus cecilia (Geoffroy in Fourcroy, 1785), with distributional notes on G. flavipes and G. ubadschii Schmidt, 1953. Entomol. News 119:81-89. DOI: https://doi.org/10.3157/0013-872X(2008)119[81:TNROOG]2.0.CO;2
Hardersen S, 2008. Dragonfly (Odonata) communities at three lotic sites with different hydrological characteristics. Ital. J. Zool 75:271-283. DOI: https://doi.org/10.1080/11250000801925227
Heidemann H, Seidenbusch R, 1993. [Die Libellenlarven Deutschlands und Frankreichs].[Book in German]. Verlag Erna Bauer, Keltern: 391 pp.
Hering D, Johnson RK, Kramm S, Schmutz S, Szoszkiewicz K, Verdonschot PFM, 2006a. Assessment of European rivers with diatoms, macrophytes, invertebrates and fish: a comparative metric-based analysis of organism response to stress. Freshwater Biol. 51:1757-1785. DOI: https://doi.org/10.1111/j.1365-2427.2006.01610.x
Hering D, Feld CK, Moog O, Ofenböck T, 2006b. Cook book for the development of a multimetric index for biological condition of aquatic ecosystems: experiences from the European AQEM and STAR projects and related initiatives. Hydrobiologia 566:311-324. DOI: https://doi.org/10.1007/s10750-006-0087-2
Horning CE, Pollard JE, 1978. Macroinvertebrate sampling techniques for streams in semi-arid regions: Comparison of the Surber method and unit-effort traveling Kick method. Report number EPA 600-4-78-040, US EPA, Washington: 37 pp.
Hughes RM, Larsen DP, Omemik JM, 1986. Regional reference sites: a method for assessing stream pollution. Environ. Manage. 10:629-635. DOI: https://doi.org/10.1007/BF01866767
Illies J, 1978. Limnofauna Europaea. Stuttgart, Gustav Fischer Verlag: 532 pp.
Janecek BFU, Moog O, Waringer J, 1995. Odonata. In: O. Moog (ed.), Fauna Aquatica Austriaca, Lieferungen 1995, 2002. Vienna, Wasserwirtschaftskataster, Bundesministerium für Land- und Forstwirtschaft, Umwelt und Wasserwirtschaft.
Johansson F, Brodin T 2003. Effects of fish predators and abiotic factors on dragonfly community structure. J. Freshwat. Ecol. 18:415–423. DOI: https://doi.org/10.1080/02705060.2003.9663977
Kalkman V, Boudot J, Bernard R, De Knijf G, Suhling F, Termaat T, 2018. Diversity and conservation of European dragonflies and damselflies (Odonata). Hydrobiologia 811:269-282. DOI: https://doi.org/10.1007/s10750-017-3495-6
Kalyoncu H, Salur A, 2018. Odonata fauna of Alara and Karpuz streams and their relations with physico-chemical variables. Fresenius Environ. Bull. 27:187-195.
King KW, Smiley Jr PC, Fausey NR, 2009. Hydrology of channelized and natural headwater streams. Hydrol. Sci. J. 54:929–948. DOI: https://doi.org/10.1623/hysj.54.5.929
Kerovec M, Ternjej I, 2017. [Study of systematic testing of biological quality elements in surface inland waters in 2016].[in Croatian] Faculty of Science, University of Zagreb: 50 pp.
Májeková M, Paal T, Plowman NS, Bryndová M, Kasari L, Norberg A, Weiss M, Bishop TR, Luke SH, Sam K, Le Bagousse-Pinguet Y, Lepš J, Götzenberger L, de Bello F. 2016. Evaluating functional diversity: missing trait data and the importance of species abundance structure and data transformation. PLoS One11:e0149270. DOI: https://doi.org/10.1371/journal.pone.0149270
May ML, 2019. Odonata: who they are and what they have done for us lately: classification and ecosystem services of dragonflies. Insects 10:1-17. DOI: https://doi.org/10.3390/insects10030062
McKinney ML, 2006. Urbanization as a major cause of biotic homogenization. Biol. Conserv. 127:247–260. DOI: https://doi.org/10.1016/j.biocon.2005.09.005
McPeek MA, 2008. Ecological factors limiting the distributions and abundances of Odonata, p. 51-62. In: A. Córdoba-Aguilar (ed.), Dragonflies: Model organisms for ecological and evolutionary research. Oxford University Press, Oxford. DOI: https://doi.org/10.1093/acprof:oso/9780199230693.003.0005
Mihaljević Z, 2011. [Revision of Croatian indicator system]. In: Z. Mihaljević (ed.), [Testing of biological methods for ecological status assessment (Water Framework Directive 2000/60/EC) in representative river basins of the Pannonian and Dinaric ecoregions]. [in Croatian]. Faculty of Science, University of Zagreb.
Moog O, 2002. Fauna Aquatica Austriaca. Vienna, Wassserwirtschaftskataster, Bundesministerium für Land- und Forstwirtschaft, Umwelt und Wasserwirtschaft.
Osborn R, 2005. Odonata as indicators of habitat quality at lakes in Louisiana, United States. Odonatologica 34:259–270.
Poff NL, Allan JD, Bain MB, Karr JR, Prestegaard KL, Richter BD, Sparks RE, Stromberg JC, 1997. The natural flow regime. BioScience 47:769–784. DOI: https://doi.org/10.2307/1313099
Raab R, 1998. [Die Libellen- und Vogelfauna im Waldviertel, p. 9-46]. In: Österreich WWF (ed.), [Natura 2000 im Waldviertel. Faunistische Erhebungen und Managementpläne im Rahmen eines LIFE-Projektes].[Book in German]. Vienna, Österreich WWF.
Raebel EM, Merckx T, Riordan P, Macdonald DW, Thompson DJ, 2010). The dragonfly delusion: why it is essential to sample exuviae to avoid biased surveys. J. Insect Conserv. 14: 523–534. DOI: https://doi.org/10.1007/s10841-010-9281-7
Rehfeldt G, 1983. [Die Libellen (Odonata) des nördlichen Harzrandes].[Article in German]. Braunschw. Naturkdl. Schr. 1:603–654.
Rose C, Crumpton WG, 1996. Effects of emergent macrophytes on dissolved oxygen dynamics in a prairie pothole wetland. Wetlands 16:495–502. DOI: https://doi.org/10.1007/BF03161339
Samways MJ, McGeoch MA, New TR, 2009. Insect conservation: handbook of approaches and methods. Oxford University Press, Oxford: 432 pp.
Samways MJ, Steytler NS, 1996. Dragonfly (Odonata) distribution patterns in urban and forest landscapes, and recommendations for riparian management. Biol. Conserv. 78:279–288. DOI: https://doi.org/10.1016/S0006-3207(96)00032-8
Sato M, Riddiford N, 2007. A preliminary study of the Odonata of S’Albufera Natural Park, Mallorca Natural Park, Mallorca: status, conservation priorities and bio-indicator potential. J. Insect Conserv. 12:539–548. DOI: https://doi.org/10.1007/s10841-007-9094-5
Schmedtje U, Colling M, 1996. [Ökologische Typisierung der aquatischen Makrofauna].[Book in German]. Informationsberichte des Bayerischen Landesamtes für Wasserwirtschaft: 543 pp.
Shi Y, Xu L, Gong D, Lu J, 2010. Effects of sterilization treatments on the analysis of TOC in water samples. J. Environ. Sc. 22:789-795. DOI: https://doi.org/10.1016/S1001-0742(09)60178-9
Silva DP, De Marco PJr, Resende DC, 2010. Adult odonate abundance and community assemblage measures as indicators of stream ecological integrity: A case study. Ecol. Indic.10:744–752. DOI: https://doi.org/10.1016/j.ecolind.2009.12.004
Solimini AG, Tarallo GA, Carchini G, 1997. Life history and species composition of the damselfly assemblage along the urban tract of a river in central Italy. Hydrobiologia 356:21–32. DOI: https://doi.org/10.1023/A:1003123129478
Steytler NS, Samways MJ, 1995. Biotope selection by adult male dragonflies (Odonata) at an artificial lake created for insect conservation in South Africa. Biol. Conserv. 72:381– 86. DOI: https://doi.org/10.1016/0006-3207(94)00052-R
Stoddard JL, Herlihy AT, Peck DV, Hughes RM, Whittier TR, Tarquinio E, 2008. A process for creating multimetric indices for large-scale aquatic surveys. J. N. Am. Benthol. Soc. 27:878–891. DOI: https://doi.org/10.1899/08-053.1
Strayer DL, Dudgeon D. 2010. Freshwater biodiversity conservation: recent progress and future challenges. J. N. Am. Benthol. Soc. 29:344–58. DOI: https://doi.org/10.1899/08-171.1
Šegota T, Filipčić A, 2003. [Köppen's division of climate and Croatian terminology].[Article in Croatian]. Geoadria 8:17.23. DOI: https://doi.org/10.15291/geoadria.93
ter Braak CJF, Smilauer P, 2012. Canoco reference Manual and user’s Guide: Software for ordination, Version 5.0. Microcomputer Power, Ithaca, NY.
TIBCO Software Inc., 2017. Statistica (data analysis software system), version 13. Available from: http://statistica.io
Tsui MTK, Blum JD, Kwon SY, Finlay JC, Balogh SJ, Nollet YH, 2012. Sources and transfers of methylmercury in adjacent river and forest food webs. Environ. Sci. Technol. 46:10957-10964. DOI: https://doi.org/10.1021/es3019836
Urbanič G, 2014. Hydromorphological degradation impact on benthic invertebrates in large rivers in Slovenia. Hydrobiologia 729:191–207. DOI: https://doi.org/10.1007/s10750-012-1430-4
Usseglio-Polatera P, Beisel J-N, 2002. Longitudinal changes in macroinvertebrate assemblages in the Meuse River: anthropogenic effects versus natural change. River Res. Appl. 18:197-211. DOI: https://doi.org/10.1002/rra.651
Vilenica M, Previšić A, Ivković M, Popijač A, Vučković I, Kučinić M, Kerovec M, Gattolliat J-L, Sartori M, Mihaljević Z, 2016. Mayfly (Insecta: Ephemeroptera) assemblages of a regulated perennial Mediterranean river system in the Western Balkans. Biologia 71:1038–1048. DOI: https://doi.org/10.1515/biolog-2016-0121
Vilenica M, Mičetić Stanković V, Sartori M, Kučinić M, Mihaljević Z, 2017. Environmental factors affecting mayfly assemblages in tufa-depositing habitats of the Dinaric Karst. Knowl. Manag. Aquat. Ec. 418:1–12. DOI: https://doi.org/10.1051/kmae/2017005
Vilenica M, Vučković N, Mihaljević Z, 2019. Littoral mayfly assemblages in South-East European man-made lakes. J. Limnol. 78:1853. DOI: https://doi.org/10.4081/jlimnol.2019.1853
Vilenica M, Pozojević I, Vučković N, Mihaljević Z, 2020. How suitable are man-made water bodies as habitats for Odonata? Knowl. Manag. Aquat. Ec. 42:1–10. DOI: https://doi.org/10.1051/kmae/2020008
Wen L, Rogers K, Ling J, Saintilan N, 2011. The impacts of river regulation and water diversion on the hydrological droughts characteristics in the Lower Murrumbidgee River, Australia. J. Hydrol. 405:382–391. DOI: https://doi.org/10.1016/j.jhydrol.2011.05.037
Williams EB, Chumchal MM, Drenner RW, Kennedy JH, 2017. Seasonality of odonate-mediated methylmercury flux from permanent and semipermanent ponds and potential risk to red-winged blackbirds (Agelaius phoeniceus). Environ. Toxicol. Chem. 36:2833–2837. DOI: https://doi.org/10.1002/etc.3844
Zaninović K, Gajić-Čapka M, Perčec Tadić M, Vučetić M, Milković J, Bajić A, Cindrić K, Cvitan L, Katušin Z, Kaučić D, Likso T, Lončar E, Lončar Ž, Mihajlović D, Pandžić K, Patarčić M, Srnec L, Vučetić V, 2008. [Climate atlas of Croatia 1961-1990, 1971-2000].[in Croatian]. Zagreb, State Hydrometeorological Institute: 200 pp.
Zhang Z, Huang J, Gang Y, Hong H, 2004. Occurrence of PAHs, PCBs and organochlorine pesticides in the Tonghui river of Beijing, China. Environ. Pollut. 130:249-261. DOI: https://doi.org/10.1016/j.envpol.2003.12.002

Edited by

Federico Marrone, Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, Italy

How to Cite

Vilenica, Marina, Mladen Kerovec, Ivana Pozojević, and Zlatko Mihaljević. 2020. “Odonata Assemblages in Anthropogenically Impacted Lotic Habitats”. Journal of Limnology 80 (1). https://doi.org/10.4081/jlimnol.2020.1968.

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