Experimental test on the use of MS-222 for ostracod anaesthesia: concentration, immersion period and recovery time

Submitted: 6 December 2011
Accepted: 6 December 2011
Published: 1 August 2010
Abstract Views: 1593
PDF: 751
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Anaesthesia of animals may be useful for different purposes, particularly for veterinary reasons or in experimental research, for manipulation or treatment of immobilized but alive animals. Its use in crustaceans is not uncommon, but it has never been described for Ostracoda. We provide brief and preliminary guidelines on the use of the tricaine mesylate (MS-222) on the widespread freshwater ostracod Eucypris virens and we show that this compound is an effective anaesthetic used as a bath treatment at minimum concentrations of 500 mg L-1. This value is considerably higher than that recommended for other aquatic animals like fish. Recovery time, ranging from 5 to 15 minutes, is mostly determined by anaesthetic bath concentration, while bath duration influenced to a lesser extent. Anaesthesia induced with MS-222 can prove useful for minute manipulation of living ostracods e.g. for identification, marking or image capture under the microscope.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

How to Cite

SCHMIT, Olivier, and Francesc MEZQUITA. 2010. “Experimental Test on the Use of MS-222 for Ostracod Anaesthesia: Concentration, Immersion Period and Recovery Time”. Journal of Limnology 69 (2):350-52. https://doi.org/10.4081/jlimnol.2010.350.

List of Cited By :

Crossref logo