Relationship between anti-oxidant capacity and manganese accumulation in the soft tissues of two freshwater molluscs: Unio pictorum mancus (Lamellibranchia, Unionidae) and Viviparus ater (Gastropoda, Prosobranchia)

Abstract

Manganese is an element of great importance in the life cycle of plants and animals. For example, it plays an essential role as an activator of various enzymatic systems such as isoenzymes of superoxide dismutase. Freshwater Unionidae concentrate relatively large amounts of manganese in their tissues, but little is known about the physiological role of this metal. The aim of this research is to acquire a better knowledge of the role of manganese in molluscs which accumulate large amounts of this metal and in those with low manganese concentrations. As manganese is one of the metals present in the superoxide molecule, the possible relationship between manganese concentration in the soft tissues of molluscs and the antioxidant capacity of the metal can usefully be tested. To this end two species of molluscs were analysed: Unio pictorum mancus (Lamellibranchia, Unionidae), which is very rich in manganese, and Viviparus ater (Gastropoda, Prosobranchia) which has a low manganese content. The adults of both species were analysed for manganese concentration by ICP, and for antioxidant capacity as RAC (Relative Antioxidant Capacity) by a superoxide dismutase method. The results clearly demonstrate the active role played by manganese against free radicals and consequently the important role of the metal in protecting Unio against oxidative stress. The low concentration of manganese in Viviparus may be the result of the effective excretion of this metal, as was found for ruthenium.