Cover Image

Are antioxidant capacity and oxidative damage related to biological and autecological characteristics in aquatic insects?

Ana Sanz, Manuel J. López-Rodríguez, Sergio García-Mesa, Cristina Trenzado, Rosa M. Ferrer, J. Manuel Tierno de Figueroa
  • Ana Sanz
    University of Granada, Department of Zoology, Spain
  • Manuel J. López-Rodríguez
    University of Granada, Department of Ecology, Spain
  • Sergio García-Mesa
    University of Granada, Department of Zoology, Spain
  • Cristina Trenzado
    University of Granada, Department of Cell Biology, Spain
  • Rosa M. Ferrer
    University of Granada, Department of Cell Biology, Spain

Abstract

In this work, we study the oxidative state of nine taxa of aquatic insects [Serratella ignita (Poda, 1761), Ephemera danica Müller, 1764, Crocothemis erythraea (Brullé, 1832), Dinocras cephalotes (Curtis, 1827), Perla bipunctata Pictet, 1833, Isoperla morenica Tierno de Figueroa & Luzón-Ortega, 2011, Notonecta maculata Fabricius, 1794, Gerris sp., and Hydropsyche sp.] in order to determine the relationships between this state and the biological and environmental characteristics of the species studied. The studied taxa are all in the same life cycle stage (nymph/larva), and many of them have different maximum life span potentials. We assess the antioxidant capacity through the determination of the trolox-equivalent antioxidant capacity, the ferric-reducing/antioxidant power and the activity of the antioxidant enzymes catalase, DT-diaphorase, glutathione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase and glucose-6-phosphate dehydrogenase. Furthermore, to determine the oxidative damage, we examine thiobarbituric acid reactive substances, free malondialdehyde, protein-bound malondialdehyde, total hydroperoxides, and protein hydroperoxides. In summary, we can consider that having predatory feeding habits, having a long-life cycle and living in permanent streams with cold, well-oxygenated waters are related to a proper oxidative state in the insects that we studied. On the other hand, non-exclusive predator species living in temporary streams with warm and poorly oxygenated waters with a short life cycle have a worse oxidative state. Thus, the oxidative state of each species could be defined by an interaction of biological and autecological factors, for which the relative importance is difficult to assess.

Keywords

Antioxidant enzymes; Feeding habits; Freshwater insects; Habitat; Life cycle; Oxidative state

Full Text:

PDF
HTML
Submitted: 2016-09-15 18:45:23
Published: 2016-11-07 00:00:00
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
1510

Views:
PDF
371
HTML
764

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2016 Ana Sanz, Manuel J. López-Rodríguez, Sergio García-Mesa, Cristina Trenzado, Rosa M. Ferrer, J. Manuel Tierno de Figueroa

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2017     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185