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Effect of starvation and subsequent feeding on glycogen concentration, behavior and mortality in the golden mussel Limnoperna fortunei (Dunker, 1857) (Bivalvia: Mytilidae

Nelmara I.S. Cordeiro, Jennifer T.M. Andrade, Lângia C. Montresor, Dalva M.R. Luz, Carlos B. Martinez, Gustavo Darrigran, Jairo Pinheiro, Teofânia H.D.A. Vidigal
  • Nelmara I.S. Cordeiro
    Universidade Federal de Minas Gerais, Brazil
  • Jennifer T.M. Andrade
    Universidade Federal de Minas Gerais, Brazil
  • Lângia C. Montresor
    FIOCRUZ, Brazil
  • Dalva M.R. Luz
    LELf Centro de Pesquisas Hidráulicas e Recursos Hídricos, Brazil
  • Carlos B. Martinez
    Universidade Federal de Minas Gerais, Brazil
  • Gustavo Darrigran
    Consejo Nacional de Investigaciones Cientificas y Tecnicas-Argentina – CONICET, Argentina
  • Jairo Pinheiro
    Universidade Federal Rural do Rio de Janeiro, Brazil

Abstract

The success of Limnoperna fortunei as an invasive species is related to its physiological plasticity that allows them to endure adverse environmental conditions. Starvation tolerance is considered to be an important trait associated with bivalve invasiveness. In natural ecosystems, food resources can vary during the year, exposing mussels to variable periods of starvation or limited food availability. Thus, mussels have developed physiological strategies to tolerate and survive fluctuations in food availability. Glycogen concentration has been used in different monitoring studies as an indicator of the nutritional condition of bivalves. The aim of this study was to investigate the physiological responses of L. fortunei based on the glycogen concentrations of specimens under four treatments, comprising different combinations of feeding and starvation, during 125 days. The experiment was carried out in two phases. In the phase I, mussels were divided in two treatments: starvation (S) and feeding (F). After 100 days, tissue samples were collected to quantify glycogen concentrations and, each phase I group was divided in two subgroups: starvation (S) and feeding (F), resulting in four treatments. In the phase II, that lasted 25 days, starvation specimens (S) from phase I were allowed to feed (starvation-feeding treatment , or S-F), or continued to undergo starvation (starvation-starvation treatment , or S-S) and the feeding specimens (F) continued feeding (feeding-feeding group, or F-F), or were subjected to starvation (feeding-starvation treatment , or F-S). Behavior (valve-closing) and mortality were recorded in 24 h intervals. After the 25 days (phase II) all specimens were killed, and their soft tissue was removed to quantify glycogen concentrations. The glycogen concentration of the S-F treatment was lower than that of the F-S treatment, which was initially allowed to feed (phase I) and then subjected to starvation (phase II). Stability in the glycogen concentrations was observed when the phase II feeding conditions were maintained during the experiments, as observed in the S-S (continued starvation) and F-F (continued feeding) treatments. Based on our glycogen concentrations results, the golden mussel shows a higher tolerance to starvation (125 days) than has previously been published, which suggests that its tolerance strongly influences its invasive behavior.

Keywords

Bioinvasion; glycogen; golden mussel; starvation; comparative physiology.

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Submitted: 2016-03-17 16:19:50
Published: 2016-07-13 00:00:00
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Copyright (c) 2016 Nelmara I.S. Cordeiro, Jennifer T.M. Andrade, Lângia C. Montresor, Dalva M.R. Luz, Carlos B. Martinez, Gustavo Darrigran, Jairo Pinheiro, Teofânia H.D.A. Vidigal

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